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Human CRISPR Knockout Pooled Library (GeCKO v2)
现货销售 人sgRNA文库 5000元, 50μg质粒A库+B库，也可单独购买单库
| 名称 | 人全基因组敲除质粒文库A | 人全基因组敲除质粒文库B |
| 注释 | Addgene：Library#1000000048; Human CRISPR Knockout Pooled Library(GeCKO v2) | |
| sgRNA数 | 65383 | 58028 |
| 覆盖基因 | 19050 Gene+1864miRNA | 19050Gene |
| 规格 | 50μg质粒A库+B库，也可单独购买单库 | |
| 文库分类 | 质粒文库 | |
| 文库功能 | CRISPR-KO | |
| 空载体 | 慢病毒单载体LentiCRISPRv2 | |
| 抗性 | Amp | |
| 筛选 | Puro | |
| 感受态 | Stbl3 | |
Purpose
The human GeCKO (Genome-Scale CRISPR Knockout) lentiviral pooled libraries target early consecutive exons for genome editing.
Vector Backbone
lentiCRISPR v2 (Plasmid #52961) backbone (one-vector system) - expresses Cas9
lentiGuide-Puro (Plasmid #52963) backbone (two-vector system) - does not express Cas9
Depositing Labs
Publication
Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.(How to cite)
| Item | Catalog # | Description | Quantity | Price (USD) | |||
|---|---|---|---|---|---|---|---|
| Pooled Library | 1000000048 | Human CRISPR KO Library (GeCKO v2) in lentiCRISPR v2 | 1 | $640 | Add to Cart | ||
| Pooled Library | 1000000049 | Human CRISPR KO Library (GeCKO v2) in lentiGuide-Puro + lentiCas9-Blast plasmid | 1 | $640 | Add to Cart | ||
Available to academic and nonprofits only.
Species
Human
Genes targeted
19,050
gRNAs
123,411
Controls
1,000 per half-library
Lentiviral Generation
3rd
Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
This library will be delivered as two pooled DNA half-libraries in microcentrifuge tubes labeled A and B.
Volume
∼20 µL
Concentration
50 ng/µL
For the two-vector system ONLY: You will also receive a bacterial stab of the lentiCas9-Blast plasmid (Addgene #52962). Please do NOT order this plasmid in addition to this library.
Protocols
For the most up-to-date and detailed instructions on using this library, please see the protocol paper from Joung, et al.
gRNA Cloning in lentiCRISPR v1, lentiCRISPR v2, and lentiGuide-Puro Vectors (PDF, 2.4 MB)
Depositor Data
Academic/Nonprofit Terms
Industry Terms
Trademarks
Zeocin® is an InvivoGen trademark.
Not Available to Industry
